%0 Journal Article %1 maniMitochondrialProteinImport2015a %A Mani, Jan %A Desy, Silvia %A Niemann, Moritz %A Chanfon, Astrid %A Oeljeklaus, Silke %A Pusnik, Mascha %A Schmidt, Oliver %A Gerbeth, Carolin %A Meisinger, Chris %A Warscheid, Bettina %A Schneider, André %C England %D 2015 %J Nature communications %K Biological Complex Evolution,Blotting Fluorescence,Mitochondrial Import Line,Kinetoplastida/genetics,Mass Membrane Northern,Carrier Precursor Protein Proteins,Phylogeny,to_read,Trypanosoma Proteins/*genetics/metabolism,Cell Proteins/*genetics/metabolism,Mitochondrial Spectrometry,Microscopy Transport brucei brucei/*genetics/metabolism %P 6646 %R 10.1038/ncomms7646 %T Mitochondrial Protein Import Receptors in Kinetoplastids Reveal Convergent Evolution over Large Phylogenetic Distances. %V 6 %X Mitochondrial protein import is essential for all eukaryotes and mediated by hetero-oligomeric protein translocases thought to be conserved within all eukaryotes. We have identified and analysed the function and architecture of the non-conventional outer membrane (OM) protein translocase in the early diverging eukaryote Trypanosoma brucei. It consists of six subunits that show no obvious homology to translocase components of other species. Two subunits are import receptors that have a unique topology and unique protein domains and thus evolved independently of the prototype receptors Tom20 and Tom70. Our study suggests that protein import receptors were recruited to the core of the OM translocase after the divergence of the major eukaryotic supergroups. Moreover, it links the evolutionary history of mitochondrial protein import receptors to the origin of the eukaryotic supergroups.

@article{maniMitochondrialProteinImport2015a, abstract = {Mitochondrial protein import is essential for all eukaryotes and mediated by hetero-oligomeric protein translocases thought to be conserved within all eukaryotes. We have identified and analysed the function and architecture of the non-conventional outer membrane (OM) protein translocase in the early diverging eukaryote Trypanosoma brucei. It consists of six subunits that show no obvious homology to translocase components of other species. Two subunits are import receptors that have a unique topology and unique protein domains and thus evolved independently of the prototype receptors Tom20 and Tom70. Our study suggests that protein import receptors were recruited to the core of the OM translocase after the divergence of the major eukaryotic supergroups. Moreover, it links the evolutionary history of mitochondrial protein import receptors to the origin of the eukaryotic supergroups.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {England}, author = {Mani, Jan and Desy, Silvia and Niemann, Moritz and Chanfon, Astrid and Oeljeklaus, Silke and Pusnik, Mascha and Schmidt, Oliver and Gerbeth, Carolin and Meisinger, Chris and Warscheid, Bettina and Schneider, Andr{\'e}}, biburl = {https://www.bibsonomy.org/bibtex/2bab4402d19ce0d8ed978bb3f463f5f5b/warscheidlab}, doi = {10.1038/ncomms7646}, interhash = {6e2dfd5e9667188c233e3da39f5395e5}, intrahash = {bab4402d19ce0d8ed978bb3f463f5f5b}, issn = {2041-1723}, journal = {Nature communications}, keywords = {Biological Complex Evolution,Blotting Fluorescence,Mitochondrial Import Line,Kinetoplastida/genetics,Mass Membrane Northern,Carrier Precursor Protein Proteins,Phylogeny,to_read,Trypanosoma Proteins/*genetics/metabolism,Cell Proteins/*genetics/metabolism,Mitochondrial Spectrometry,Microscopy Transport brucei brucei/*genetics/metabolism}, langid = {english}, month = mar, pages = 6646, pmcid = {PMC4389251}, pmid = {25808593}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {Mitochondrial Protein Import Receptors in {{Kinetoplastids}} Reveal Convergent Evolution over Large Phylogenetic Distances.}, volume = 6, year = 2015 }