%0 Journal Article %1 richter-dennerleinMitochondrialProteinSynthesis2016 %A Richter-Dennerlein, Ricarda %A Oeljeklaus, Silke %A Lorenzi, Isotta %A Ronsör, Christin %A Bareth, Bettina %A Schendzielorz, Alexander Benjamin %A Wang, Cong %A Warscheid, Bettina %A Rehling, Peter %A Dennerlein, Sven %C United States %D 2016 %J Cell %K 1/biosynthesis/genetics/*metabolism,cytochrome Active Cell Cells,Humans,Membrane Complex IV/biosynthesis/genetics/*metabolism,HEK293 Line Messenger/biosynthesis/genetics,RNA Mitochondrial,to_read,translation Mitochondrial/genetics,Electron Nucleus,assembly,C12ORF62,Cell Phosphorylation,OXPHOS,Ribosomes/metabolism,RNA Proteins/biosynthesis/genetics/*metabolism,Mitochondria/*enzymology,Mitochondrial Proteins/biosynthesis/genetics/*metabolism,mitochondrial Transport Tumor,COX1,Cyclooxygenase c oxidase,DNA plasticity regulation,translational ribosome,mitochondrial translation,MITRAC,Oxidative %N 2 %P 471-483.e10 %R 10.1016/j.cell.2016.09.003 %T Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein. %V 167 %X Mitochondrial ribosomes translate membrane integral core subunits of the oxidative phosphorylation system encoded by mtDNA. These translation products associate with nuclear-encoded, imported proteins to form enzyme complexes that produce ATP. Here, we show that human mitochondrial ribosomes display translational plasticity to cope with the supply of imported nuclear-encoded subunits. Ribosomes expressing mitochondrial-encoded COX1 mRNA selectively engage with cytochrome c oxidase assembly factors in the inner membrane. Assembly defects of the cytochrome c oxidase arrest mitochondrial translation in a ribosome nascent chain complex with a partially membrane-inserted COX1 translation product. This complex represents a primed state of the translation product that can be retrieved for assembly. These findings establish a mammalian translational plasticity pathway in mitochondria that enables adaptation of mitochondrial protein synthesis to the influx of nuclear-encoded subunits.

@article{richter-dennerleinMitochondrialProteinSynthesis2016, abstract = {Mitochondrial ribosomes translate membrane integral core subunits of the oxidative phosphorylation system encoded by mtDNA. These translation products associate with nuclear-encoded, imported proteins to form enzyme complexes that produce ATP. Here, we show that human mitochondrial ribosomes display translational plasticity to cope with the supply of imported nuclear-encoded subunits. Ribosomes expressing mitochondrial-encoded COX1 mRNA selectively engage with cytochrome c oxidase assembly factors in the inner membrane. Assembly defects of the cytochrome c oxidase arrest mitochondrial translation in a ribosome nascent chain complex with a partially membrane-inserted COX1 translation product. This complex represents a primed state of the translation product that can be retrieved for assembly. These findings establish a mammalian translational plasticity pathway in mitochondria that enables adaptation of mitochondrial protein synthesis to the influx of nuclear-encoded subunits.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {United States}, author = {{Richter-Dennerlein}, Ricarda and Oeljeklaus, Silke and Lorenzi, Isotta and Rons{\"o}r, Christin and Bareth, Bettina and Schendzielorz, Alexander Benjamin and Wang, Cong and Warscheid, Bettina and Rehling, Peter and Dennerlein, Sven}, biburl = {https://www.bibsonomy.org/bibtex/2b63a96062872d6c66da6956674abd704/warscheidlab}, copyright = {Copyright {\copyright} 2016 The Author(s). Published by Elsevier Inc. All rights reserved.}, doi = {10.1016/j.cell.2016.09.003}, interhash = {251a83dda30dfe88d5a8147fc44b7df0}, intrahash = {b63a96062872d6c66da6956674abd704}, issn = {1097-4172 0092-8674}, journal = {Cell}, keywords = {1/biosynthesis/genetics/*metabolism,cytochrome Active Cell Cells,Humans,Membrane Complex IV/biosynthesis/genetics/*metabolism,HEK293 Line Messenger/biosynthesis/genetics,RNA Mitochondrial,to_read,translation Mitochondrial/genetics,Electron Nucleus,assembly,C12ORF62,Cell Phosphorylation,OXPHOS,Ribosomes/metabolism,RNA Proteins/biosynthesis/genetics/*metabolism,Mitochondria/*enzymology,Mitochondrial Proteins/biosynthesis/genetics/*metabolism,mitochondrial Transport Tumor,COX1,Cyclooxygenase c oxidase,DNA plasticity regulation,translational ribosome,mitochondrial translation,MITRAC,Oxidative}, langid = {english}, month = oct, number = 2, pages = {471-483.e10}, pmcid = {PMC5055049}, pmid = {27693358}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {Mitochondrial {{Protein Synthesis Adapts}} to {{Influx}} of {{Nuclear-Encoded Protein}}.}, volume = 167, year = 2016 }