%0 Journal Article %1 Young2010Phosphatidic %A Young, Barry P. %A Shin, John J. %A Orij, Rick %A Chao, Jesse T. %A Li, Shu Chen C. %A Guan, Xue Li L. %A Khong, Anthony %A Jan, Eric %A Wenk, Markus R. %A Prinz, William A. %A Smits, Gertien J. %A Loewen, Christopher J. %D 2010 %J Science (New York, N.Y.) %K biosensor metabolism %N 5995 %P 1085--1088 %R 10.1126/science.1191026 %T Phosphatidic acid is a pH biosensor that links membrane biogenesis to metabolism. %U http://dx.doi.org/10.1126/science.1191026 %V 329 %X Recognition of lipids by proteins is important for their targeting and activation in many signaling pathways, but the mechanisms that regulate such interactions are largely unknown. Here, we found that binding of proteins to the ubiquitous signaling lipid phosphatidic acid (PA) depended on intracellular pH and the protonation state of its phosphate headgroup. In yeast, a rapid decrease in intracellular pH in response to glucose starvation regulated binding of PA to a transcription factor, Opi1, that coordinately repressed phospholipid metabolic genes. This enabled coupling of membrane biogenesis to nutrient availability.

@article{Young2010Phosphatidic, abstract = { Recognition of lipids by proteins is important for their targeting and activation in many signaling pathways, but the mechanisms that regulate such interactions are largely unknown. Here, we found that binding of proteins to the ubiquitous signaling lipid phosphatidic acid ({PA}) depended on intracellular {pH} and the protonation state of its phosphate headgroup. In yeast, a rapid decrease in intracellular {pH} in response to glucose starvation regulated binding of {PA} to a transcription factor, Opi1, that coordinately repressed phospholipid metabolic genes. This enabled coupling of membrane biogenesis to nutrient availability. }, added-at = {2018-12-02T16:09:07.000+0100}, author = {Young, Barry P. and Shin, John J. and Orij, Rick and Chao, Jesse T. and Li, Shu Chen C. and Guan, Xue Li L. and Khong, Anthony and Jan, Eric and Wenk, Markus R. and Prinz, William A. and Smits, Gertien J. and Loewen, Christopher J.}, biburl = {https://www.bibsonomy.org/bibtex/220929183fd96b4e90cacdb16a2b2c51c/karthikraman}, citeulike-article-id = {7750969}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1191026}, citeulike-linkout-1 = {http://www.sciencemag.org/content/329/5995/1085.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/329/5995/1085.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/20798321}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=20798321}, day = 27, doi = {10.1126/science.1191026}, interhash = {5988ea123ed34a7ca57e58119138b325}, intrahash = {20929183fd96b4e90cacdb16a2b2c51c}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {biosensor metabolism}, month = aug, number = 5995, pages = {1085--1088}, pmid = {20798321}, posted-at = {2010-09-01 09:29:12}, priority = {5}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Phosphatidic acid is a {pH} biosensor that links membrane biogenesis to metabolism.}, url = {http://dx.doi.org/10.1126/science.1191026}, volume = 329, year = 2010 }